In vitro effect of ananas comosus extract on human sperm

  • Mélany Uribe-Clavijo Universidad de Antioquia, Medellín
  • Ángela M. Álvarez-Gómez Universidad de Antioquia, Medellín
  • Víctor Arango Universidad de Antioquia, Medellín
  • Fabian M. Cortes-Mancera Instituto Tecnológico Metropolitano (ITM), Medellín
  • Ángela P. Cadavid-Jaramillo Universidad de Antioquia, Medellín
  • Walter D. Cardona-Maya Universidad de Antioquia, Medellín
Keywords: Spermatozoa, spermicides, Ananas comosus, contraception, hyperactivation.

Abstract

Spermicides has been used for birth control, but its chemical components are harmful for the vaginal epithelium, thus other studies have been done, given the possibility to use different extracts from plants as spermicidal agents that avoid vaginal tissue damages. The aim of this study was to evaluate the Ananas comosus extract as spermicidal. Semen samples were incubated with pineapple extract, and motility and viability was evaluated after contact. In addition the cytotoxic effect was evaluated using MTS assay on HeLa cells. Motility (p<0.001) and viability (p<0.001) decreased after treatment with 50% dilution. However, extract had a cytotoxic effect on epithelial cell line. In conclusion, despite the cytotoxic effect in the same concentrations that spermicidal activity was observed, further studies determining the fractions of the extract have spermicidal properties are necessary.

 

Author Biographies

Mélany Uribe-Clavijo, Universidad de Antioquia, Medellín
Grupo Reproducción, Facultad de Medicina,
Universidad de Antioquia, Medellín
Ángela M. Álvarez-Gómez, Universidad de Antioquia, Medellín
Grupo Reproducción, Facultad de Medicina,
Universidad de Antioquia, Medellín
Víctor Arango, Universidad de Antioquia, Medellín
Grupo Productos Marinos,
Universidad de Antioquia, Medellín
Fabian M. Cortes-Mancera, Instituto Tecnológico Metropolitano (ITM), Medellín
Grupo de Investigación en Innovación Biomédica SINERGIA, Facultad de Ciencias Exactas y Aplicadas, Instituto Tecnológico Metropolitano (ITM), Medellín
Ángela P. Cadavid-Jaramillo, Universidad de Antioquia, Medellín
Grupo Reproducción, Facultad de Medicina,
Universidad de Antioquia, Medellín
Walter D. Cardona-Maya, Universidad de Antioquia, Medellín
Grupo Reproducción, Facultad de Medicina,
Universidad de Antioquia, Medellín
How to Cite
Uribe-Clavijo, M., Álvarez-Gómez, Ángela M., Arango, V., Cortes-Mancera, F. M., Cadavid-Jaramillo, Ángela P., & Cardona-Maya, W. D. (2012). In vitro effect of ananas comosus extract on human sperm. TecnoLógicas, (28), 55-70. https://doi.org/10.22430/22565337.13

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Published
2012-06-25
Section
Research Papers

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