Microfabrication of position reference patterns onto glass microscope slides for high-accurate analysis of dynamic cellular events

  • July A. Galeano Instituto Tecnológico Metropolitano
  • Patrick Sandoz University Bourgogne Franche-Comté
  • Artur Zarzycki Instituto Tecnológico Metropolitano
  • Laurent Robert University Bourgogne Franche-Comté
  • Juan M. Jaramillo Universidad EAFIT
Keywords: Microtechnology, lift-off process, pseudo-periodic patterns, glass microscope slides, micropatterning


Glass microscopes slides are widely used as in situ base-substrates carrying diverse micro-fabricated systems or elements. For such purposes, the micro-fabrication process consists in transferring a pre-defined design onto the substrate made of a glass microscope slide. This is known as patterning, which is a technique that can also be used in transferring specific designs that allows region of interest (ROI) recovery under the microscope. In those cases, two main challenges appear: 1) Disturbances in light transmission should remain minimum to keep the high quality of observation of the object of interest under the microscope. 2) The pattern-size should then be small enough but, however, larger than the diffraction limit to be observable satisfactorily for positioning purposes. In this article, we present the procedures involved in the microfabrication of Pseudo-Periodic Patterns (PPP) encrypting the absolute position of an extended area. Those patterns are embedded in Pétri dishes in order to allow the highaccurate retrieval of absolute position and orientation. The presented microfabrication is based in a technique known as lift-off, which after parameter adjustment, allows the obtaining of PPP fulfilling the two previously mentioned requirements. The results report on PPP realized on glass microscope slides and composed by 2µm side dots made of aluminum with a thickness of 30nm.

Author Biographies

July A. Galeano, Instituto Tecnológico Metropolitano

PhD Engineering, Ingeniería de Sistemas, Grupo de Investigación en Materiales Avanzados y Energía MatyEr, Línea Biomateriales y Electromedicina, Facultad de Ingenierías

Patrick Sandoz, University Bourgogne Franche-Comté

PhD Chargé de Recherche C.N.R.S., Department of Applied Mechanics, FEMTO-ST Institute

Artur Zarzycki, Instituto Tecnológico Metropolitano

PhD Engineering, Ingeniería Electromecánica, Grupo de Investigación en Automática, Electrónica y Ciencias Computacionales, Línea Sistemas de Control y Robótica, Facultad de Ingenierías

Laurent Robert, University Bourgogne Franche-Comté

PhD Ingénieur de Recherche, FEMTO-ST Institute

Juan M. Jaramillo, Universidad EAFIT

PhD Engineering, Grupo de Investigación Electromagnetismo Aplicado, Línea Microingeniería


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Microfabrication of position reference patterns onto glass microscope slides for high-accurate analysis of dynamic cellular events
TecnoLógicas, ISSN 0123-7799 - ISSN-e 2256-5337, Vol. 20, No. 39, mayo - agosto de 2017
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How to Cite
Galeano, J. A., Sandoz, P., Zarzycki, A., Robert, L., & Jaramillo, J. M. (2017). Microfabrication of position reference patterns onto glass microscope slides for high-accurate analysis of dynamic cellular events. TecnoLógicas, 20(39), 115-126. https://doi.org/10.22430/22565337.695


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