Microfabrication of position reference patterns onto glass microscope slides for high-accurate analysis of dynamic cellular events

  • July A. Galeano Instituto Tecnológico Metropolitano
  • Patrick Sandoz University Bourgogne Franche-Comté
  • Artur Zarzycki Instituto Tecnológico Metropolitano
  • Laurent Robert University Bourgogne Franche-Comté
  • Juan M. Jaramillo Universidad EAFIT
Keywords: Microtechnology, lift-off process, pseudo-periodic patterns, glass microscope slides, micropatterning


Glass microscopes slides are widely used as in situ base-substrates carrying diverse micro-fabricated systems or elements. For such purposes, the micro-fabrication process consists in transferring a pre-defined design onto the substrate made of a glass microscope slide. This is known as patterning, which is a technique that can also be used in transferring specific designs that allows region of interest (ROI) recovery under the microscope. In those cases, two main challenges appear: 1) Disturbances in light transmission should remain minimum to keep the high quality of observation of the object of interest under the microscope. 2) The pattern-size should then be small enough but, however, larger than the diffraction limit to be observable satisfactorily for positioning purposes. In this article, we present the procedures involved in the microfabrication of Pseudo-Periodic Patterns (PPP) encrypting the absolute position of an extended area. Those patterns are embedded in Pétri dishes in order to allow the highaccurate retrieval of absolute position and orientation. The presented microfabrication is based in a technique known as lift-off, which after parameter adjustment, allows the obtaining of PPP fulfilling the two previously mentioned requirements. The results report on PPP realized on glass microscope slides and composed by 2µm side dots made of aluminum with a thickness of 30nm.


Download data is not yet available.

Author Biographies

July A. Galeano, Instituto Tecnológico Metropolitano

PhD Engineering, Ingeniería de Sistemas, Grupo de Investigación en Materiales Avanzados y Energía MatyEr, Línea Biomateriales y Electromedicina, Facultad de Ingenierías

Patrick Sandoz, University Bourgogne Franche-Comté

PhD Chargé de Recherche C.N.R.S., Department of Applied Mechanics, FEMTO-ST Institute

Artur Zarzycki, Instituto Tecnológico Metropolitano

PhD Engineering, Ingeniería Electromecánica, Grupo de Investigación en Automática, Electrónica y Ciencias Computacionales, Línea Sistemas de Control y Robótica, Facultad de Ingenierías

Laurent Robert, University Bourgogne Franche-Comté

PhD Ingénieur de Recherche, FEMTO-ST Institute

Juan M. Jaramillo, Universidad EAFIT

PhD Engineering, Grupo de Investigación Electromagnetismo Aplicado, Línea Microingeniería


[1] R. Ramji, N. T. Khan, A. Muñoz-Rojas, and K. Miller-Jensen, “‘Pop-slide’ patterning: rapid fabrication of microstructured PDMS gasket slides for biological applications,” RSC Adv., vol. 5, no. 81, pp. 66294–66300, 2015. [2] B. Gumuscu, A. den Berg, and J. C. T. Eijkel, “Custom micropatterning of hydrogels in closed microfluidic platforms fabricated by capillary pinning,” in The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2014. [3] J. Lafaurie-Janvore, E. E. Antoine, S. J.
Microfabrication of position reference patterns onto glass microscope slides for high-accurate analysis of dynamic cellular events
TecnoLógicas, ISSN 0123-7799 - ISSN-e 2256-5337, Vol. 20, No. 39, mayo - agosto de 2017
Perkins, A. Babataheri, and A. I. Barakat, “A simple microfluidic device to study cell-scale endothelial mechanotransduction,” Biomed. Microdevices, vol. 18, no. 4, p. 63, Aug. 2016. [4] N. Nagarajan, K. Hung, and P. Zorlutuna, “Protein Micropatterning Techniques for Tissue Engineering and Stem Cell Research,” in Cell and Material Interface: Advances in Tissue Engineering, Biosensor, Implant, and Imaging Technologies, vol. 52, CRC Press, 2015, pp. 109–146. [5] J. Jaramillo, A. Zarzycki, J. Galeano, and P. Sandoz, “Performance Characterization of an xy-Stage Applied to Micrometric Laser Direct Writing Lithography.,” Sensors (Basel)., vol. 17, no. 2, p. 278, Jan. 2017. [6] C.-T. Chen, “Inkjet Printing of Microcomponents: Theory, Design, Characteristics and Applications,” in Features of Liquid Crystal Display Materials and Processes, InTech, 2011, pp. 43–60. [7] K. Boolchandani and B. Sarita, “A Review Paper on Nanotechnology Applications and Concepts,” in IJIRST || National Conference on Innovations in Microelectronics, Signal Processing and Communication Technologies (V-IMPACT2016), 2016, pp. 61–62. [8] A. Nag, A. I. Zia, S. C. Mukhopadhyay, and J. Kosel, “Performance enhancement of electronic sensor through mask-less lithography,” in 2015 9th International Conference on Sensing Technology (ICST), 2015, pp. 374–379. [9] D. St-Jacques, S. Martel, and T. B. FitzGerald, “Nanoscale Grid based potitioning system for miniature instrumented robots,” in Canadian Conference on Electrical and Computer Engineering, 2003, vol. 3, pp. 1831–1834. [10] D. B. Boyton, “Position encoder using statistically biased pseudorandom sequence.” 2004.
[11] V. Guelpa, P. Sandoz, M. A. Vergara, C. Clévy, N. Le Fort-Piat, and G. J. Laurent, “2D visual micro-position measurement based on intertwined twin-scale patterns,” Sensors Actuators A Phys., vol. 248, pp. 272– 280, Sep. 2016. [12] M. J. Yao, “Method of printing location markings on surfaces for microscopic research.” 2013. [13] M. Wrenn and D. Soenksen, “Systems and methods for tracking a slide using a composite barcode label.” 2016. [14] J.-A. Galeano-Zea, P. Sandoz, E. Gaiffe, J.-L. Pretet, and C. Mougin, “Pseudo-Periodic Encryption of Extended 2-D Surfaces for High Accurate Recovery of any Random Zone by Vision,” Int. J. Optomechatronics, vol. 4, no. 1, pp. 65–82, Jan. 2010. [15] J. A. Galeano Z., P. Sandoz, E. Gaiffe, S. Launay, L. Robert, M. Jacquot, F. Hirchaud, J.-L. Prétet, and C. Mougin, “Positionreferenced microscopy for live cell culture monitoring,” Biomed. Opt. Express, vol. 2, no. 5, p. 1307, May 2011. [16] M. GmbH, Lithography: Theory and Applications of Photoresists, Developers, Solvents and Etchants. MicroChemicals GmbH, 2007. [17] M. J. Madou, Manufacturing techniques for microfabrication and nanotechnology, 1st ed., vol. 2. Boca Ratón - Florida: CRC Press, 2011. [18] Elveflow, “How to do PDMS lithography replication from a su-8 mold: The PDMS lithography replication process: tips and tricks,” The SU-8 mold fabrication process: tips and tricks. [Online]. Available: http://www.elveflow.com/microfluidictutorials/soft-lithography-reviews-andtutorials/introduction-in-softlithography/pdms-softlithographyreplication/.
How to Cite
Galeano, J., Sandoz, P., Zarzycki, A., Robert, L., & Jaramillo, J. (2017). Microfabrication of position reference patterns onto glass microscope slides for high-accurate analysis of dynamic cellular events. TecnoLógicas, 20(39), 115-126. https://doi.org/10.22430/22565337.695
Research Papers

Most read articles by the same author(s)