Evaluación de la actividad bactericida de GO electroquímico modificado con nanopartículas de TiO2
Resumen
Recientemente, los materiales antibacterianos han despertado un renovado interés en el campo de la ingeniería biomédica y las ciencias de la vida. El objetivo de este estudio consistió en evaluar las propiedades fisicoquímicas de nanopartículas de TiO2 fase anatasa y un tamaño medio de 24.1 ± 4.6 nm, óxido de grafeno (GO) obtenido a partir del método electroquímico y un nanomaterial hibrido TiO2/GO. El análisis termogravimétrico (TGA) reveló la presencia de funcionalidades de oxígeno en la estructura del GO, y se encontró un 23.2 % de TiO2 en el nanomaterial híbrido y una fuerte interacción entre los materiales que puede observarse en las micrografías de microscopia electrónica de barrido (SEM). Las pruebas antibacterianas fueron realizadas usando el método de macrodilución. Los resultados evidenciaron que, mientras que el GO no disminuyó el crecimiento bacteriano, el TiO2 presentó una alta actividad bactericida. A su vez, el nanomaterial híbrido TiO2/GO no mostró dicha actividad. Este resultado puede explicarse por la disminución del contacto entre el TiO2 y las células bacterianas debido al bloqueo de los sitios activos en la superficie del TiO2 por las láminas de óxido de grafeno. Estos resultados contribuyen a la discusión en curso sobre las propiedades bactericidas del óxido de grafeno.
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