Evaluación de andamios microestructurados 3D con base en PCL/fibroína/nanopartículas de plata como soportes para células de piel
Resumen
La ingeniería de tejidos de la piel es un campo en el cual se utilizan células vivas y andamios para tratar defectos. Esta investigación tuvo como objetivo fabricar y evaluar andamios tridimensionales microestructurados a partir de policaprolactona (PCL), fibroína de seda y nanopartículas de plata (Ag-NP) mediante la técnica de wet-electrospinning. La metodología empleada consistió en extraer la fibroína de capullos de Bombyx Mori y se sintetizaron Ag-NP mediante reducción química, combinándolas con soluciones de PCL para crear andamios 3D. Estos se caracterizaron a través de microscopía electrónica de barrido (SEM), análisis térmico (TGA y DSC), espectroscopía FTIR y pruebas de ángulo de contacto al agua (WCA). Adicionalmente, se evaluó su citocompatibilidad mediante el ensayo MTT utilizando la línea celular de fibroblastos de ratón L929. Los resultados mostraron que la inclusión de fibroína y Ag-NP mejoró la hidrofília y la citocompatibilidad de los andamios cumpliendo con la norma ISO 10993-5:2009. La técnica de wet-electrospinning permitió obtener estructuras porosas con propiedades térmicas y morfológicas adecuadas para imitar la matriz extracelular. Finalmente, se concluye que los andamios desarrollados muestran una alta viabilidad para su uso como sustrato para la regeneración de tejidos cutáneos, subrayando la necesidad de realizar estudios in vivo que respalden su aplicación en entornos clínicos.
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Datos de los fondos
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Instituto Tecnológico Metropolitano
Números de la subvención P20209
